Journal: Communications Biology

Article Title: SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2

doi: 10.1038/s42003-026-09678-9

Figure Lengend Snippet: A IOD of ACAT2 expression in CC tissues and adjacent tissues was examined using immunohistochemical staining ( n = 47 biologically independent samples). IOD of DHCR7 B and MSMO1 C expression in CC patients with high ( n = 27 biologically independent samples) or low ( n = 20 biologically independent samples) expression of ACAT2 was examined using immunohistochemical staining. The number of activated CD8 T cells (CD8A + GZMB + ) D or activated NK cells (CD56 + GZMB + ) E infiltrated in the tumor tissues of patients with high ( n = 27 biologically independent samples) and low ACAT2 ( n = 20 biologically independent samples) expression was detected. Data represent mean ± SEM. Statistical analysis was performed using the paired A or unpaired ( B – E ) t-test.

Article Snippet: The cell suspension (100 μL) was incubated with BeyoFC Fc Receptor Blocking Solution (C1755, Beyotime) for 10 min at 4 °C and with primary antibodies, including FITC-coupled CD3 antibody (1:100, FITC-65077, ProteinTech, RRID: AB_2883763), PE-coupled NK1.1 antibody (1:100, PE-65138, ProteinTech, RRID: AB_2883920), and APC-coupled CD8A antibody (1:100, APC-65069, ProteinTech, RRID: AB_2882970) for 1 h at 4 °C.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Communications Biology

Article Title: SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2

doi: 10.1038/s42003-026-09678-9

Figure Lengend Snippet: ACAT2 expression in HCeEpiC and CC cell lines was examined using RT-qPCR A and Western blot analysis B ( n = 5 independent experiments). C ACAT2, DHCR7, and MSMO1 expression in CC cells after infection with Scramble-sh, ACAT2-sh #1, and ACAT2-sh #2 was examined using Western blot analysis ( n = 5 independent experiments). D Detection of total cholesterol, free cholesterol, and cholesteryl ester levels in CC cells ( n = 5 independent experiments). The proliferation of CC cells was examined using CCK8 ( E ) and colony formation assays F (n = 5 independent experiments). G CC cells were co-cultured with (E: T = 3:1) with NK cells or CD8 T cells for 6 h, respectively, and the death of CC cells was detected ( n = 5 independent experiments). H IFN-γ and GZMB released from immune cells in a co-culture system with CC cells were examined using ELISA ( n = 5 independent experiments). Data represent mean ± SEM. Statistical analysis was performed using the one-way ( A , B ) or two-way ( C - H ) ANOVA, followed by Tukey’s multiple comparisons test ( A – H ).

Article Snippet: The cell suspension (100 μL) was incubated with BeyoFC Fc Receptor Blocking Solution (C1755, Beyotime) for 10 min at 4 °C and with primary antibodies, including FITC-coupled CD3 antibody (1:100, FITC-65077, ProteinTech, RRID: AB_2883763), PE-coupled NK1.1 antibody (1:100, PE-65138, ProteinTech, RRID: AB_2883920), and APC-coupled CD8A antibody (1:100, APC-65069, ProteinTech, RRID: AB_2882970) for 1 h at 4 °C.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Infection, Cell Culture, Co-Culture Assay, Enzyme-linked Immunosorbent Assay

Journal: Communications Biology

Article Title: SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2

doi: 10.1038/s42003-026-09678-9

Figure Lengend Snippet: A ACAT2 knockdown efficiency in U14 cells was examined using western blot analysis ( n = 10 independent experiments). B Volume changes of transplanted tumors in mice subcutaneously inoculated with U14 cells (n = 10 animals). C The images and weight of the tumors harvested on day 21 ( n = 10 animals). D Protein expression of ACAT2, MSMO1, DHCR7, and PCNA in transplanted tumors was examined using western blot analysis ( n = 10 animals). E Detection of total cholesterol, free cholesterol, and cholesteryl ester levels in transplanted tumors ( n = 10 animals). The gating strategy for GZMB + NK cells and CD8 + T cells F and quantification G were analyzed using flow cytometry ( n = 10 animals). H Survival of mice over 60 days after subcutaneous inoculation of U14 cells was analyzed using the log-rank test ( n = 20 animals). Data represent mean ± SEM. Statistical analysis was performed using the one-way ( A , C , E , G ) or two-way ( B , D ) ANOVA, followed by Tukey’s multiple comparisons test.

Article Snippet: The cell suspension (100 μL) was incubated with BeyoFC Fc Receptor Blocking Solution (C1755, Beyotime) for 10 min at 4 °C and with primary antibodies, including FITC-coupled CD3 antibody (1:100, FITC-65077, ProteinTech, RRID: AB_2883763), PE-coupled NK1.1 antibody (1:100, PE-65138, ProteinTech, RRID: AB_2883920), and APC-coupled CD8A antibody (1:100, APC-65069, ProteinTech, RRID: AB_2882970) for 1 h at 4 °C.

Techniques: Knockdown, Western Blot, Expressing, Flow Cytometry

Journal: Communications Biology

Article Title: SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2

doi: 10.1038/s42003-026-09678-9

Figure Lengend Snippet: The proliferation of CC cells was examined using CCK8 A and colony formation assays B ( n = 5 independent experiments). C CC cells were co-cultured with (E: T = 3:1) with NK cells or CD8 + T cells, and the death of CC cells was detected ( n = 5 independent experiments). D IFN-γ and GZMB released from immune cells in a co-culture system with CC cells were examined using ELISA ( n = 5 independent experiments). E TGF-β1 released by CC cells was examined using ELISA ( n = 5 independent experiments). F PD-L1 expression levels in CC cells were observed using immunofluorescence staining ( n = 5 independent experiments). Data represent mean ± SEM. Statistical analysis was performed using the two-way ( A – F ) ANOVA, followed by Tukey’s multiple comparisons test.

Article Snippet: The cell suspension (100 μL) was incubated with BeyoFC Fc Receptor Blocking Solution (C1755, Beyotime) for 10 min at 4 °C and with primary antibodies, including FITC-coupled CD3 antibody (1:100, FITC-65077, ProteinTech, RRID: AB_2883763), PE-coupled NK1.1 antibody (1:100, PE-65138, ProteinTech, RRID: AB_2883920), and APC-coupled CD8A antibody (1:100, APC-65069, ProteinTech, RRID: AB_2882970) for 1 h at 4 °C.

Techniques: Cell Culture, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining

Journal: Communications Biology

Article Title: SREBF2 enhances lipid metabolism and represses anti-tumor immune responses in cervical cancer by increasing ACAT2

doi: 10.1038/s42003-026-09678-9

Figure Lengend Snippet: A Volume changes of transplanted tumors in mice subcutaneously inoculated with U14 cells ( n = 5 animals). B The images and weight of the tumors harvested on day 21 ( n = 5 animals). The gating strategy for GZMB + NK cells and CD8 + T cells C and quantification D were analyzed using flow cytometry ( n = 5 animals). Data represent mean ± SEM. Statistical analysis was performed using the one-way ( B , D ) or two-way A ANOVA, followed by Tukey’s multiple comparisons test.

Article Snippet: The cell suspension (100 μL) was incubated with BeyoFC Fc Receptor Blocking Solution (C1755, Beyotime) for 10 min at 4 °C and with primary antibodies, including FITC-coupled CD3 antibody (1:100, FITC-65077, ProteinTech, RRID: AB_2883763), PE-coupled NK1.1 antibody (1:100, PE-65138, ProteinTech, RRID: AB_2883920), and APC-coupled CD8A antibody (1:100, APC-65069, ProteinTech, RRID: AB_2882970) for 1 h at 4 °C.

Techniques: Flow Cytometry

Journal: Nature Communications

Article Title: Papilloma-pseudovirus eradicates intestinal tumours and triples the lifespan of Apc Min/+ mice

doi: 10.1038/ncomms15004

Figure Lengend Snippet: ( a ) Survival of hCEA-Tg/Apc Min/+ mice that were immunized (one round of three-dose immunization) with VLP-GFP or VLP-hCEA. One group of mice were pretreated with anti-CD8a antibody to deplete CD8 + T cells before VLP-hCEA immunization ( n =9–14, including 5–7 male and 4–7 female mice per group). Data are representative of two independent experiments. ( b ) Percentage of survival of Apc Min/+ ( n =11, including 7 male and 4 female mice) or hCEA-Tg/Apc Min/+ ( n =10, including 6 male and 4 female mice) mice that were immunized (one round of three-dose immunization) with VLP-hCEA. Data are representative of three independent experiments. ( c ) The percentage of survival of Apc Min/+ mice that were immunized (one round of three-dose immunization) with VLP-hCEA or VLP-GFP. Two groups of mice were pretreated with anti-CD8a antibody before VLP-hCEA or VLP-GFP immunization ( n =9–14, including 5–8 male and 4–7 female mice per group). Data are representative of two independent experiments. Log-rank test was performed to determine the statistical significance in ( a – c ).

Article Snippet: For CD8 + T-cell depletion, mice were i.p. injected with 250 μg of anti-CD8a IgG2a antibody (from Cedarlane) at 2 days and 1 day before every pseudovirus immunization.

Techniques:

Journal: Nature immunology

Article Title: Cytotoxic innate lymphoid cells sense cancer cell-expressed interleukin-15 to suppress human and murine malignancies.

doi: 10.1038/s41590-022-01213-2

Figure Lengend Snippet: Fig. 1 | chRCC and ccRCC tumors exhibit differential immune cell infiltration and CD8 T cell phenotypes. a, t-Distributed stochastic neighbor embedding (tSNE) of transcriptional profiles from leukocytes isolated from one chRCC tumor and one ccRCC tumor. Each dot represents a single CD45+ cell, and colors represent clusters denoted by cell type inferred from lineage markers and differential gene expression. b, tSNE plot as in a, colored by histology (chromophobe or clear cell). c, For each CD8 cluster, the frequency out of all CD8α+ clusters at which it was found in chRCC (n = 1) and ccRCC (n = 1) tumors. d, Violin plots showing log-normalized expression of selected differentially expressed genes (DEGs) among three CD8 clusters. e, Representative plots of flow cytometric analysis of the percentage of CD3+CD8α+ T cells out of the lymphocyte gate (CD45+SSCLow) in blood, adjacent normal kidney and tumor samples from one patient of the indicated histology. Quantification is CD3+CD8α+ T cells out of total CD45+ cells. f, Representative histograms of PD-1 expression in CD3+CD8α+ T cells from blood, adjacent normal kidney and tumor tissues from a single patient of the indicated histology. Quantification of flow cytometric analysis of percentage of PD-1+CD3+CD8α+ T cells in blood, adjacent normal kidney and tumor samples from the indicated histology. e,f, Each pair of symbols connected by a line denotes an individual patient (chRCC blood n = 6 (e and f), kidney n = 9 (e) and 10 (f), tumor n = 9 (e) and 10 (f); ccRCC blood n = 14, kidney n = 15 and tumor n = 16). One-way analysis of variance (ANOVA) with Tukey’s multiple comparison test was used for statistical analysis (NS, not significant; **P < 0.01, ****P < 0.0001.

Article Snippet: Antihuman CD8a (RPA-T8, #25-0088-T100) and biotinylated anti-human CD3 (UCHT1, #30-0038-U100) were purchased from Tonbo Biosciences.

Techniques: Isolation, Gene Expression, Expressing, Comparison

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Immune cell infiltration in KRAS G12C mutant PNETs (A) Quantitative analysis using Image Plus 6.0 software reveals a significant increase in Tregs (CD4 + , CD25 + , and FoxP3 + ) within KRAS- G12C mutant PNETs compared to wild-type KRAS PNET tissues, suggesting KRAS G12C -driven immunosuppressive cell recruitment. (B) Fluorescence intensity analysis demonstrates reduced infiltration of CD8 + cytotoxic T cells and HLA-DR + activated cells in KRAS G12C mutant tumors, indicative of impaired antitumor immunity. (C) Elevated MDSCs counts in KRAS G12C mutant PNETs correlate with enhanced immune evasion.

Article Snippet: APC Anti-Human CD8a Antibody [OKT-8] , Elabscience , E-AB-F1110E.

Techniques: Mutagenesis, Software, Fluorescence

Journal: iScience

Article Title: Hypoxic-immune axis orchestrates metastatic dissemination via HIF isoform imbalance in pancreatic neuroendocrine tumors

doi: 10.1016/j.isci.2025.114340

Figure Lengend Snippet: Tregs, CD8 + T cells, and HLA-DR + cells in KRAS G12C -mutated PNETs (A) Flow cytometry plots and fluorescence intensity histograms demonstrate elevated CD4 + T cell proportions in KRAS G12C patient blood samples compared to wild-type KRAS tumors and healthy controls. (B) Quantification shows a significant enrichment of CD25 + T cells in KRAS G12C patients, surpassing both wild-type KRAS tumors and normal controls. (C) Quantitative data and histogram overlays confirm a substantial increase in FoxP3 + T cells frequency in KRAS G12C patients, with levels moderately elevated compared to wild-type KRAS and significantly higher than healthy individuals. (D) A slight decrease in CD8 + T cell frequency in KRAS G12C samples relative to wild-type KRAS, with levels significantly lower than those in healthy individuals (E) A moderate reduction in HLA-DR + cell frequency in KRAS G12C patients compared to wild-type KRAS, alongside a marked suppression relative to healthy controls.

Article Snippet: APC Anti-Human CD8a Antibody [OKT-8] , Elabscience , E-AB-F1110E.

Techniques: Flow Cytometry, Fluorescence

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Reconstitution of Circulating Mucosal-Associated Invariant T Cells after Allogeneic Hematopoietic Cell Transplantation: Its Association with the Riboflavin Synthetic Pathway of Gut Microbiota in Cord Blood Transplant Recipients.

doi: 10.4049/jimmunol.1900681

Figure Lengend Snippet: FIGURE 3. Comparison of MR1 tetramer+ MAIT cells and CD161+TCR Va7.2+MAIT cells in long-term survivors after allogeneic HCT. (A) Gating strategies for CD161+TCR Va7.2+MAIT cells and MR1: 5-OP-RU and 6-FP tetramer+ MAIT cells. (B) Correlation of proportion of MR1: 5-OP-RU tetramer+ MAIT cells, CD161+TCR Va7.2+ MAIT cells, CD4+CD161+T cells, CD8+CD161+T cells, and MR-1: 6-FP tetramer+ MAIT cells among CD3+

Article Snippet: To examine the proportion of MAIT cells, PBMCs were stained with allophycocyanin-Cy7-conjugated anti-human CD3 (clone SK7; BioLegend, San Diego, CA), FITC-conjugated anti-human TCR g/d (clone B1; BioLegend), PE-conjugated anti-human CD161 (clone HP-3G10; Tonbo Biosciences, San Diego, CA), BV421-conjugated anti-human TCR Va7.2 (clone 3C10; BioLegend), BV510-conjugated anti-human CD4 (clone RPA-T4; BioLegend), PE-Cy7-conjugated anti-human CD8a (clone RPA-T8; Tonbo Biosciences), allophycocyanin-conjugated antihuman programmed cell death–1 (PD-1) (clone EH12.2H7; BioLegend), and PerCP/Cy5.5-conjugated anti-human TCR Va24-Ja18 (clone 6B11; BioLegend) Abs.

Techniques: Comparison

Journal: Drug delivery and translational research

Article Title: Self-assembled peptide/polymer hybrid nanoplatform for cancer immunostimulating therapies.

doi: 10.1007/s13346-023-01410-y

Figure Lengend Snippet: Fig. 1 Scheme of the technological strategy: peptide diblocks consisting of PADRE/MAGE-A3 are self-assembled as nanoparticles. These immunoactive nanoparticles can interact with dendritic cells to activate CD4 + and CD8 + T-cells capable of killing cancer cells

Article Snippet: Anti CD4-APC, anti CD25-PE, anti CD8a-APC, and anti CD28-PE antibodies were obtained from Tonbo Biosciences (California, USA).

Techniques: